EIF4EBP1 Antibody, KO Validated | 14-450

EIF4EBP1 Antibody, KO Validated | 14-450 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1) .

Research Area: Obesity, Signal Transduction

Tested Application: WB, IHC, IF

Application: WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
IF: 1:100 - 1:200

Specificiy: N/A

Positive Control 1: MCF7

Positive Control 2: HeLa

Positive Control 3: SH-SY5Y

Positive Control 4: NIH/3T3

Positive Control 5: Mouse fat

Positive Control 6: Mouse heart

Molecular Weight: Observed: 17kDa

Validation: Antibody is Knockout validated.

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: 4E-BP1 Antibody: BP-1, 4EBP1, 4E-BP1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E) , which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.