ELAVL1 Antibody [AMM22119G]

ELAVL1 Antibody [AMM22119G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This ELAVL1 monoclonal antibody is generated from mouse immunized with ELAVL1 recombinant protein.

Cellular Localisation: Cytoplasm. Nucleus. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:P70372}. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288) Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462) .Localizes to the stress granules in the presence of PLEKHN1 (By similarity) {ECO:0000250|UniProtKB:P70372, ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:18285462}

Molecular Weight: 36092

Clone: Monoclonal

Gene Name: ELAVL1

Gene ID: 1994

Function: RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969) . Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity) . Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:14731398) . Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503) . With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity) . Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010) .

Summary: Tissue Location: Ubiquitous. Detected in brain, liver, thymus and muscle.

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IF, WB

Dilution: WB--1:1000 IF--1:10~50

Synonyms: ELAV-like protein 1, Hu-antigen R, HuR, ELAVL1, HUR