ENDOG polyclonal Antibody | BS60425

ENDOG polyclonal Antibody | BS60425 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1) . This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA (ssDNA) . Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ENDOG polyclonal Antibody detects endogenous levels of ENDOG protein.

Molecular Weight: ~ 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Endonuclease G, mitochondrial; Endo G

Immunogen: A synthetic peptide corresponding to residues in Human ENDOG.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: