EndoPDI Antibody | 45-536

EndoPDI Antibody | 45-536 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: N/A

Immunogen: The immunogen for this antibody is: C-SLHRFVLSQAKDEL

Research Area: Cancer

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6kDa according to NP_110437.2) . The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TXNDC5, EndoPDI, thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide, thioredoxin domain containing 5 (endoplasmic reticulum) isomerase, Hcc-2, PDIA15, endothelial protein disulphide isomerase, protein disulfide isomerase family A, member 15, thioredoxin domain containing 5

User Note: N/A

BACKGROUND: N/A