Enterokinase, Light Chain, human recombinant | 7529

Enterokinase (EC 3.4.21.9) is a serine protease involved in the activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.

7529 | Enterokinase Light Chain human recombinant DataSheet

Biomolecule/Target: Enterokinase

Synonyms: Enteropeptidase, Serine protease 7, Transmembrane protease serine 15

Alternates names: IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23

Taglines: Activates trypsinogen to trypsin.

NCBI Gene ID #: 50616

NCBI Gene Symbol: IL22

Gene Source: Human

Accession #: Q9GZX6

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Recombinant IL-22 activates STAT following receptor-ligand interaction.

Results: >12,000 mU/mg

Binding Capacity: N/A

Unit Definition: 1 U = Cleavage of 1 µmole per min of Ac-DDDDK-AFC at 37°C in 50 mM Tris, 1 mM CaCl2, 0.1% Tween 20, pH 8.0

Molecular Weight: 16.8 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 25 mM Tris, 50 mM NaCl (pH 8.0) containing 50% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum. This solution can then be stored at 4°C for 1 week or -20° for future use.

Amino acid sequence: N/A