Eotaxin-3 Antibody [1.1_3H10-1D11] | 38-238

Eotaxin-3 Antibody [1.1_3H10-1D11] | 38-238 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Eotaxin-3 antibody is produced in BALB/c ICRR F1 mice using highly pure (>98%) recombinant human Eotaxin-3 as the immunizing antigen.

Research Area: Immunology, Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL) , a concentration of 1.0-5.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL.
Western Blot:
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.

Clonality: Monoclonal

Clone: 1.1_3H10-1D11

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: Eotaxin-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: SCYA26, UNQ216/PRO242, C-C motif chemokine 26, CC chemokine IMAC, MIP-4-alpha, IMAC, TSC-1, MIP-4a, MIP-4alpha

User Note: Centrifuge vial prior to opening.

BACKGROUND: N/A