Eotaxin Antibody [1.3_17H09-02C06] | 38-234

Eotaxin Antibody [1.3_17H09-02C06] | 38-234 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Eotaxin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin as the immunizing antigen.

Research Area: Immunology, Chemokines & Cytokines

Tested Application: E, WB

Application: ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Clonality: Monoclonal

Clone: 1.3_17H09-02C06

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: Eotaxin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: SCYA11, Eotaxin, C-C motif chemokine 11SCYA11

User Note: Centrifuge vial prior to opening.

BACKGROUND: Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.