Epigen (EPGN), human recombinant | 4969

EPGN is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human epigen produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques.

4969 | Epigen EPGN human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.

Alternates names: GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy

Taglines: Promotes the growth of epithelial cells

NCBI Gene ID #: 2660

NCBI Gene Symbol: GDF8

Gene Source: Human

Accession #: O14793

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to inhibit the proliferation of MPC-11 cells. The ED for this effect is <20 ng/ml.

Results: 150-300 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized from 20 mM PBS buffer pH-7.4 containing 130 mM sodium chloride

Reconstitution Instructions: It is recommended to reconstitute the lyophilized human Myostatin in sterile 20 mM acetic acid to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A