EpiQuik In-situ and Ex-Situ Hydrogen Peroxide (H2O2) Assay Kit | P-6005

The EpiQuik™ In-situ and Ex-Situ Hydrogen Peroxide (H2O2) Assay Kit is a complete set of optimized buffers and reagents to accurately measure hydrogen peroxide from various samples in-situ (living cells) and ex-situ (cell/tissue extracts and body fluids) in a fast and sensitive manner. The kit has the following advantages and features:

• Fast procedure, which can be finished within 10 min.
• Homogeneous fluorescence assay for both in-situ (live cells) and ex-situ (cell/tissue extract or body fluids) H₂O₂detection, which is highly sensitive with LOD around 0.03 µM or 1 pg.
• Strip-based microplate format makes the assay flexible via manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

Background Information
Hydrogen peroxide (H₂O₂) is a reactive oxygen species generated in cellular oxygen metabolism. Abnormal H₂O₂ contributes to oxidative cell damage and the progress of diseases such as inflammation, ischemia, and neurodegeneration. Hydrogen peroxide is a major marker of the ROS release from cell or cell organelles such as mitochondria. H₂O₂ can also be an indicator of the oxidase activity such as NADPH and glucose oxidase as a co-product of oxidase-catalyzed reactions. Thus, it is important to detect H₂O₂ from various biological samples in determining how oxidative stress modulates varied intracellular pathways under physiological and pathological conditions.

Principle & Procedure
This kit is designed for the measurement of hydrogen peroxide from various samples in-situ and ex-situ. In the assay with this kit, H₂O₂ assay probe fluorogen 10-Acetyl-3,7-dihydroxy-phenoxazine is incubated with various samples in the strip wells. Hydrogen peroxide contained in or generated from the samples reacts with the H₂O₂ assay probe and produces highly fluorescent oxidation products. The measured intensity of fluorescence from oxidation products is proportional to the amount or concentration of H₂O₂.

Starting Materials
Starting materials can include live cells, extracts from various tissue or cell samples such as cells from a flask or microplate cultured cells, culture supernatant, plasma/serum, and urine samples, etc.