ERCC5 Antibody (C-term) [APR31598G]

ERCC5 Antibody (C-term) [APR31598G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ERCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1151-1178 amino acids from the C-terminal region of human ERCC5.

Cellular Localisation: Nucleus. Chromosome. Note=Colocalizes with RAD51 to nuclear foci in S phase (PubMed:26833090) . Localizes to DNA double-strand breaks (DBS) during replication stress (PubMed:26833090) . Colocalizes with BRCA2 to nuclear foci following DNA replication stress (PubMed:26833090) .

Molecular Weight: 133108

Clone: Polyclonal

Gene Name: ERCC5

Gene ID: 2073

Function: Single-stranded structure-specific DNA endonuclease involved in DNA excision repair (PubMed:8206890, PubMed:8090225, PubMed:8078765, PubMed:7651464, PubMed:32821917, PubMed:32522879) . Makes the 3'incision in DNA nucleotide excision repair (NER) (PubMed:8090225, PubMed:8078765, PubMed:32821917, PubMed:32522879) . Binds and bends DNA repair bubble substrate and breaks base stacking at the single- strand/double-strand DNA junction of the DNA bubble (PubMed:32522879) . Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA (PubMed:9927729) . Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed:16246722) . Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II (PubMed:16246722) . Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity (PubMed:16246722) . Required for DNA replication fork maintenance and preservation of genomic stability (PubMed:26833090, PubMed:32522879) . Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site (PubMed:26833090) . During HRR, binds to the replication fork with high specificity and stabilizes it (PubMed:32522879) . Also, acts upstream of HRR, to promote the release of BRCA1 from DNA (PubMed:26833090) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: DNA repair protein complementing XP-G cells, 31--, DNA excision repair protein ERCC-5, Xeroderma pigmentosum group G-complementing protein, ERCC5, ERCM2, XPG, XPGC