ERK1/2 (Phospho-T202/Y204) polyclonal Antibody | AP0484

ERK1/2 (Phospho-T202/Y204) polyclonal Antibody | AP0484 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Rat,Mouse

Application: WB IF IHC

Application Range: WB: 1:5000~1:10000 IHC: 1:50~1:200 IF: 1:50~1:200

Background: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK) . Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ERK1/2 (Phospho-T202/Y204) polyclonal Antibody detects endogenous levels of ERK1/2 protein only when phosphorylated at Thr202/Tyr204.

Molecular Weight: ~ 42,44 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2, PRKM1, PRKM2

Immunogen: Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202 / Tyrosine 204.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Immune Cell Signaling,Warburg Effect,Regulation of Microtubule Dynamics,T cell receptor signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,Mitochondrial Control of Apoptosis,ErbB HER Signaling,