Erythropoietin Antibody [EPO/1368] | 33-731

Erythropoietin Antibody [EPO/1368] | 33-731 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: A human partial recombinant protein was used as the immunogen for the EPO antibody.

Research Area: Cancer, Cell Cycle, Obesity, Signal Transduction

Tested Application: WB, IHC-P, Flow, IF

Application: WB: 0.5-1 ug/ml
IF: 1-2 ug/ml
Immunohistochemistry (FFPE) : 4-8 ug/ml for 30 min at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Titering of the EPO antibody may be required for optimal performance.

1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: EPO/1368

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Erythropoietin, Epoetin, EPO

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.