FA12 (heavy chain,Cleaved-Ile20) polyclonal Antibody | BS65134

FA12 (heavy chain,Cleaved-Ile20) polyclonal Antibody | BS65134 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB,IHC-p,IF (paraffin section),ELISA

Application Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Background: coagulation factor XII (F12) Homo sapiens This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF) -like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activat

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cleaved-Factor XII HC (I20) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XII HC protein resulting from cleavage adjacent to I20.

Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: F12; Coagulation factor XII; Hageman factor; HAF

Immunogen: The antiserum was produced against synthesized peptide derived from human FA12. AA range:1-50

Conjugate: Unconjugated

Modification: Cleaved

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pathway: