Factor Va, Human Plasma | 4098

Factor Va is a cofactor for the serine protease factor Xa, and in the presence of calcium ions they collectively assemble on a phospholipid surface to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of prothrombin to thrombin. Factor Va is derived from the pro-cofactor, factor V, upon limited proteolysis by alpha-thrombin. The thrombin cleavage of factor V liberates two heavily glycosylated activation peptides from the central portion of the molecule which have no cofactor function. Factor Va is comprised of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain associated in the presence of calcium ions. The cofactor binds to phospholipid (cell membrane) surfaces and effectively serves as a receptor for membrane bound factor Xa. Complete assembly of the prothrombinase complex (factor Xa, factor Va, phospholipid, and calcium) results in a 300,000-fold increase in the rate of prothrombin conversion relative to the rate observed with factor Xa alone. The interaction between factor Va and factor Xa is mediated by both the heavy and light chain of factor Va, while the binding of prothrombin to factor Va is mediated solely by the heavy chain.

4098 | Factor Va Human Plasma DataSheet

Biomolecule/Target: Factor Va

Synonyms: Activated protein C cofactor, Proaccelerin (labile factor)

Alternates names: bone morphogenetic protein 13, GDF-6, Cartilage-Derived Morphogenetic Protein-2, CDMP-2

Taglines: A serine protease co-ordinating multiple physiological functions

NCBI Gene ID #: 392255

NCBI Gene Symbol: GDF6

Gene Source: Human

Accession #: Q6KF10

Recombinant: No

Source: Human plasma

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): Greater than 1000 u/mg (1u= Factor V activity in 1 ml normal human plasma)

Biological activity: The ED as determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is in the range of 2-3 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 27 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Liquid in 41.3 µl 20 mM HEPES, 150 mM NaC1, pH 7.5, 5 mM CaCl2 +50% glycerol

Reconstitution Instructions: Reconstitute to 0.1-1.0 mg/ml in HO containing BSA (50 g BSA per 1 g of protein).

Amino acid sequence: N/A