Fas Antibody [APO-1-3] (preservative free) | 36-167

Fas Antibody [APO-1-3] (preservative free) | 36-167 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human Fas.

Research Area: Apoptosis, Cancer, Functional Assays

Tested Application: Flow, FUNC, IP, WB

Application: ELISA: (direct or indirect: 1:2, 000-1:10, 000) . Western blot: (1:2, 000-1:5, 000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

Specificiy: Recognizes human Fas.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: APO-1-3

Isotype: IgG3

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS. Preservative free formulation.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-kappaB.