FastAmp Plant Tissue & Seed Genotyping Kit

Description

FastAmp^® Plant Tissue & Seed Genotyping Kit is suitable for amplification of DNA directly from plant samples without purifying DNA. This kit is based on specially engineered Taq DNA polymerase, proprietary buffer system, dNTP, MgCl₂, PCR facilitators and dye mix which makes it extremely robust and tolerant of plant PCR inhibitors such as complex polysaccharides, polyphenols and others. This PCR master mix has been tested with leaves and seeds from a wide variety of plant species. This kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for high-throughput genotyping from various Plant tissue/seeds without DNA purification steps .

Highlights

• Direct PCR- no need to purify DNA
• Specially engineered Taq DNA polymerase with highest sensitivity and specificity
• Extremely short PCR protocol times
• Master mix format with premixed gel loading dye to reduce cross-contamination and sample handling
  errors
• Optimized for both low and high GC templates

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

 Product Includes

• FastAmp^® Plant Direct PCR master mix (2x)
• FastAmp^® Plant Direct PCR/Genotyping Solution

Storage Temperature
–20ºC

Quality Control Assays

FastAmp^® Plant Tissue/Seed Genotyping Kit has been tested with tissue from a wide variety of plant species, some of the results are included here.

Genotyping PCR analysis from Plant leaf tissue in 96 plate using FastAmp^® Plant Tissue/Seed Genotyping PCR kit.