FBL Antibody (Center E120) [APR15925G]

FBL Antibody (Center E120) [APR15925G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This FBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-135 amino acids from the Central region of human FBL.

Cellular Localisation: Nucleus, nucleolus. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:P35550}. Note=Fibrillar region of the nucleolus

Molecular Weight: 33784

Clone: Polyclonal

Gene Name: FBL (HGNC:3599)

Gene ID: 2091

Function: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898) . Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930) . Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity) . Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity) . Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898) . U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898) . Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: rRNA 2'-O-methyltransferase fibrillarin, 211-, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, FBL, FIB1, FLRN