FBP1, human recombinant | 7803

Fructose-1, 6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.

7803 | FBP1 human recombinant DataSheet

Biomolecule/Target: FBP1

Synonyms: Fructose-1,6-bisphosphatase 1, FBPase1, FBP, FBP1

Alternates names: Fructose-1,6-bisphosphatase 1, FBPase1, FBP, FBP1

Taglines: A gluconeogenesis regulatory enzyme

NCBI Gene ID #: 54250

NCBI Gene Symbol: FGF-2

Gene Source: Human

Accession #: P13109

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 0.30 unit/ml, in which one unit will convert 1.0 µmole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25ºC

Results: Specific activity is > 0.30 unit/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25ºC

Molecular Weight: 39.0 kDa (358 aa, 1-338 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol and 1 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ