FBXW7 Antibody (N-term) [APR33093G]

FBXW7 Antibody (N-term) [APR33093G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This FBXW7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 177-208 amino acids from the N-terminal region of human FBXW7.

Cellular Localisation: [Isoform 1]: Nucleus, nucleoplasm. Chromosome Note=Localizes to site of double-strand breaks following phosphorylation by ATM. [Isoform 3]: Nucleus, nucleolus

Molecular Weight: 79663

Clone: Polyclonal

Gene Name: FBXW7 (HGNC:16712)

Gene ID: 55294

Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:22748924, PubMed:17434132, PubMed:26976582, PubMed:28727686) . Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:22748924, PubMed:26774286, PubMed:17434132, PubMed:26976582, PubMed:28727686) . Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923, PubMed:22748924, PubMed:29149593, PubMed:25775507, PubMed:28007894, PubMed:26976582, PubMed:28727686) . Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463) . Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593) . Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF (FBXW7) -mediated ubiquitination (PubMed:27238018) . Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage (PubMed:26774286) . The SCF (FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining (PubMed:26774286) .

Summary: Tissue Location: [Isoform 1]: Widely expressed.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: F-box/WD repeat-containing protein 7, Archipelago homolog, hAgo, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4, FBXW7 (HGNC:16712)