Fc RIIa/FCGR2A/CD32a | E21-S35

Fc RIIa/FCGR2A/CD32a | E21-S35 | Gentaur UK, US & Europe Distribution

Short Description: Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-A is produced by our Mammalian expression system and the target gene encoding Ala36-Ile218 (His131Arg) is expressed with a 6His tag at the C-terminus.

Shipping: Ambient

Formulation: Lyophilized from a 0.2 m filtered solution of PBS, pH7.4.

Storage: Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 g /ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/g (1 IEU/g) as determined by LAL test.

Background: Human FcRs are divided into three classes designated FcRI (CD64) , FcRII (CD32) , and FcRIII (CD16) , which generate multiple isoforms, are recognized. The activating type receptor either has or associates noncovalently with an accessory subunit that has an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain. FcRI binds IgG with high affinity and functions during early immune responses, whereas FcRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.Human CD32, also known as Low affinity immunoglobulin Fc region receptor II-a (IgG Fc receptor II-a) , FcRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcR, CD32a (FcRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fc RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

Species: Human

Expression System: Human cells