FCSD2 Antibody (C-term) [APR15945G]

FCSD2 Antibody (C-term) [APR15945G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This FCSD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-734 amino acids from the C-terminal region of human FCSD2.

Cellular Localisation: Cytoplasm {ECO:0000250|UniProtKB:Q3USJ8}. Cell junction. Membrane, clathrin-coated pit. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, stereocilium {ECO:0000250|UniProtKB:Q3USJ8}. Note=Partially localized at clathrin-coated pits at the cell membrane (PubMed:30249660) . Detected at the cell membrane at sites around clathrin-coated pits, very close to the clathrin-coated pits but not an intrinsic part of the clathrin-coated pits (PubMed:29887380) Colocalizes at cell-cell contacts with CDH1, but is not detected at tight junctions (PubMed:14627983) .

Molecular Weight: 84276

Clone: Polyclonal

Gene Name: FCHSD2

Gene ID: 9873

Function: Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor (PubMed:29887380) . Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling (PubMed:30249660) . Recruited to clathrin-coated pits during a mid-to- late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits (PubMed:29887380) . Binds to membranes enriched in phosphatidylinositol 3, 4-bisphosphate or phosphatidylinositol 3, 4, 5-trisphosphate (PubMed:29887380) . When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerisation in the absence of membranes (PubMed:29887380) .

Summary: Tissue Location: Liver, brain, heart, placenta, skeletal muscle, pancreas, lung and kidney.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: FCH and double SH3 domains protein 2, Carom, SH3 multiple domains protein 3, FCHSD2, KIAA0769, SH3MD3