FEN1 Polyclonal Antibody | E-AB-60232

FEN1 Polyclonal Antibody | E-AB-60232 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: FEN1, FEN-1, MF1, RAD2

Application: WB, IHC, IF

Reactivity: Human, Mouse

Host: Rabbit

Isotype: IgG

Reserch Areas: Cancer, Epigenetics and Nuclear Signaling

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant fusion protein of human FEN1 (NP_004102.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:10-1:100

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 35 kDa/42 kDa

Molecular Weight(Observed): 48 kDa