Fetuin A (Human) ELISA Kit - E5041

E5041 | Fetuin A (Human) ELISA Kit DataSheet

Fetuin A is a glycoprotein which is also called as alpha-2-HS-glycoprotein. It is abundant in fetal blood and is a hepatocyte derived protein. It serves as a transport protein for calcium and phosphate, inhibits extraosseous calcification and plays an important role in bone mineralization, insulin resistance and breast tumor cell proliferative signaling. It is downregulated in Alzheimer's disease patients and could serve as a potential biomarker. ’s Fetuin A (Human) ELISA Kit is used for the quantitative detection of Fetuin A in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of Fetuin A in the sample.

Alternate Name : FETUA ELISA Kit; AHSG ELISA Kit; alpha-2-HS-glycoprotein ELISA Kit; A2HS ELISA Kit; alpha-2-Z-globulin ELISA Kit; Alpha-2-Heremans-Schmid Glycoprotein ELISA kit

Tag Line : A highly sensitive ELISA kit for quantitative measurement of Fetuin A

Summary :

Detection Method : Absorbance is measured at 450 nm

Sample Type : Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity : Human

Applications : The ELISA kit is used for the quantitative detection of Fetuin A in serum, plasma, tissue homogenates and other biological fluids

Features and Benefits : Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Recovery Rate: 90-105% for serum, 90-104% for EDTA plasma and 85-105% for heparin plasma Detection range: 0.781-50 ng/ml Highly sensitive and specific Sensitivity: 0.469 ng/ml