FGF-21, murine recombinant | 4067

The FGFs are a family of more than 20 small (~17–26 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. FGFR-2 appears to be a key molecule during pancreatic development and FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis. FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents. Fibroblast Growth Factor-21 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 183 amino acids including N-terminal Methionine and having a molecular mass of 20.1 kDa. The amino acid sequence of the recombinant mouse FGF21 is 100% homologous to the amino acid sequence of the mouse FGF21 without signal sequence. The FGF-21 is purified by proprietary chromatographic techniques.

4067 | FGF-21 murine recombinant DataSheet

Biomolecule/Target: FGF-21

Synonyms: FGF21, Fibroblast growth factor 21, FGFL

Alternates names: Parathyrin, PTH, Parathormone

Taglines: A heparin binding growth factor, stimulating the proliferation and activation of cells expressing FGFR

NCBI Gene ID #: 5741

NCBI Gene Symbol: PTH

Gene Source: Mouse

Accession #: P01270

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Recombinant human PTH is fully active when compared to standards. The activity calculated by mR10 cells/cAMP method corresponds to a specific activity of 1 x 10 IU/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 9.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from filtered (0.4 mm) solution containing 20 mM Tris, pH 7.4 and 20 mM NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A