Description
FGF-basic Antibody [20B242.2] | XP-5718-M | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hFGF-basic as the immunizing antigen.
Research Area: Growth Factors
Tested Application: E, WB
Application: ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human FGF-basic when used with our biotinylated antigen affinity purified anti-human FGF-basic (XP-5137Bt) as the detection antibody at a concentration of approximately 0.125-0.25 μg/mL.
Western blot:
To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: This IgG1/K antibody was purified from a Protein A chromatography column.
Clonality: Monoclonal
Clone: 20B242.2
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: FGF-basic lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Alternate Name: Fibroblast growth factor, FGFB, Fibroblast growth factor 2, Basic fibroblast growth factor, FGF-2, BFGF, HBGF-2
User Note: Centrifuge vial prior to opening.
BACKGROUND: N/A