FGFR3 Antibody | 7425

FGFR3 Antibody | 7425 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (95%)

Immunogen: FGFR3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FGFR3.
The immunogen is located within amino acids 630 - 680 of FGFR3.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: FGF3 antibody can be used for detection of FGFR3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: FGFR3 antibody is human, mouse and rat reactive. At least three isoforms of FGFR3 are known to exist; this antibody will detect all three.

Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 89 kDa
Observed: 9+2, 98 kDa

Validation: N/A

Isoform: N/A

Purification: FGFR3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: FGFR3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: FGFR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: FGFR3 Antibody: ACH, CEK2, JTK4, CD333, HSFGFR3EX, Fibroblast growth factor receptor 3, FGFR-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: FGFR3 Antibody: FGFR3 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis (1) . It is required for normal skeleton development and promotes apoptosis in chondrocytes and cancer cell proliferation. FGFR3 protein contains three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain (1, 2) . It is alternatively spliced to produce three isoforms that are expressed in brain, kidney and testis. Defects in FGFR3 are associated with several diseases, including achondroplasia and hypochondroplasia (3, 4) . Mutations in FGFR3 are also a cause of some bladder and cervical cancers (5) .