FGR, Active | 7724

Fgr is a protooncogene that is a unique member of the tyrosine kinase gene family. It is localized to the distal portion of the short arm of human chromosome 1 at p36.1-36.2 by in situ hybridization (1). Certain lymphomas (but not sarcomas or carcinomas) express fgr-related messenger RNA. This transcript is detected in Burkitt's lymphoma cell lines naturally infected with Epstein-Barr virus (EBV), but not in EBV-negative Burkitt's lymphoma cells (2). Normal umbilical cord or peripheral blood lymphocyte lines established in vitro by EBV infection also contain detectable c-fgr mRNA. Moreover, a 50-fold increase of the steady-state c-fgr mRNA concentration is observed when uninfected Burkitt's lymphoma cell lines are deliberately infected with EBV demonstrating the induction of a proto-oncogene in response to infection by a DNA tumour virus. Fgr expression is limited to normal peripheral blood granulocytes, monocytes, and alveolar macrophages, all of which contain 50 to 100 copies of c-fgr mRNA per cell (3). The c-fgr RNA molecules in these cells consisted of partially spliced transcripts containing intron 7 and completely spliced molecules capable of encoding the predicted p55 c-fgr protein. The level of fgr transcripts begin to increase 2 to 4 h after TPA addition, peak at 8 h, and subsequently declined suggesting transient transcriptional activation of fgr during TPA-induced differentiation. Cycloheximide also causes accumulation of c-fgr transcripts in U937 cells. Thus, c-fgr gene is expressed in a tissue- and development-specific fashion and constitutive expression of c-fgr in U937 cells is regulated by a labile transcriptional repressor.

7724 | FGR Active DataSheet

Biomolecule/Target: N/A

Synonyms: FGR, Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog

Alternates names: FGR, Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, Proto-oncogene c-Fgr, p55-Fgr, p58-Fgr, p58c-Fgr

Taglines: A negative regulator of cell migration and adhesion

NCBI Gene ID #: 281161

NCBI Gene Symbol: FGF-2

Gene Source: N/A

Accession #: P03969

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 282 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 86.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: N/A

Amino acid sequence: N/A