Fish CLDN3 (Claudin 3) ELISA Kit | ELK8519

Fish CLDN3 (Claudin 3) ELISA Kit | ELK8519 | Gentaur UK, US & Europe Distribution

Alternative Names: C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2

Species: Fish

Assay Type: Sandwich

Sensitivity: 5.65 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 3 (CLDN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Claudin 3 (CLDN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 3 (CLDN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 3 (CLDN3) in the samples is then determined by comparing the OD of the samples to the standard curve.