FitAmp Urine DNA Isolation Kit | P-1017

FitAmp Urine DNA Isolation Kit DataSheet

The FitAmp™ Urine DNA Isolation Kit is a complete set of essential components that enables researchers to efficiently isolate DNA from urine. Depending on sample types, the entire procedure can be completed within 20 minutes.

• Fast - Entire procedure can be finished in just 20 mins with consistent isolation conditions.
• Effective -High efficiency of DNA isolation from urine.
• Reliable – Our specially designed F-Spin Columns allow for DNA to be conveniently recovered.
• Safe -Use of non-toxic reagents and no phenol chloroform.

This kit can also be employed as the initial step in our popular DNA methylation & hydroxymethylation quantitation kits: MethylFlash Global DNA Methylation 5-mC ELISA Easy Kit, and the MethylFlash  Global DNA Methylation 5-hmC ELISA Easy Kit.

Background
DNA found in urine is mainly derived from cells shed into the urine from the urinary tract. DNA isolated from urine may be used in many different applications in research (ex: DNA methylation quantification) and diagnostics (ex: cancer testing). Using urine DNA would have special advantages for diagnostics, as the collection of urine is completely non-invasive and the isolation of DNA from urine is easier than from blood. The FitAmp™ Urine DNA Isolation Kit ensures ready-to-use DNA no matter what the downstream application is.

Principle & Procedure
This kit simply applies our proprietary DNA isolation buffer to urine sediments. After treatment with the DNA digestion buffer, the DNA is easily recovered in 8- 20 µl through our specially designed F-Spin Column. DNA is then ready for down-stream application.

Starting Materials
Input amount of urine can be 1 ng to 2 µg, with the optimal range between 10 ng and 1 µg. This kit can easily yield 1 ng to 4 µg of DNA in spin column format, which can be used for any molecular biology procedures such as PCR, restriction digestion, cloning and sequencing, etc.