FITC-Tyramide | B3054

Tyramide signal amplification is an enzyme-catalyzed deposition of tyramide on or near a protein or nucleic acid in situ. The enzyme horseradish peroxidase (HRP) converts the labelled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on target molecules at or near the HRP, in the presence of low concentrations of hydrogen peroxide. Labelled FITC-tyramide reagent enhances both the fluorescent and colorimetric detection sensitivity. FITC-tyramide is used in multiplex immunohostochemistry (IHC) and Fluorescent in situ hybridization (FISH). FITC-tyramide is useful for detecting marine picoplankton, in situ imaging of proteins and studying the effects of reactive oxygen species on extracellular matrix proteins.

Tyramide signal amplification is an enzyme-catalyzed deposition of tyramide on or near a protein or nucleic acid in situ. The enzyme horseradish peroxidase (HRP) converts the labelled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on target molecules at or near the HRP, in the presence of low concentrations of hydrogen peroxide. Labelled FITC-tyramide reagent enhances both the fluorescent and colorimetric detection sensitivity. FITC-tyramide is used in multiplex immunohostochemistry (IHC) and Fluorescent in situ hybridization (FISH). FITC-tyramide is useful for detecting marine picoplankton, in situ imaging of proteins and studying the effects of reactive oxygen species on extracellular matrix proteins.

B3054 | FITC-Tyramide DataSheet

Alternate Name/Synonyms: 1-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-3-(4-hydroxyphenethyl)thiourea; Fluorescein isothiocyanate-tyramide

Appearance: Orange powder

Formulation:

CAS Number: N/A

Structure Available?: TRUE

Peptide sequence:

Salt Form: FALSE

Molecular Formula: C₂₉H₂₂N₂O₆S

Molecular Weight: 526.6

Cell-Permeable?: TRUE

Purity: 0.98

Solubilities: ~30 mg/ml in ethanol, ~35 mg/ml in DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A signal amplification reagent for IHC and FISH

MDL Number:

PubChem CID:

SMILES: S=C(NCCC1=CC=C(O)C=C1)NC2=CC3=C(C4(C5=C(OC6=C4C=CC(O)=C6)C=C(O)C=C5)OC3=O)C=C2

InChi: InChI=1S/C29H22N2O6S/c32-18-4-1-16(2-5-18)11-12-30-28(38)31-17-3-8-22-21(13-17)27(35)37-29(22)23-9-6-19(33)14-25(23)36-26-15-20(34)7-10-24(26)29/h1-10,13-15,32-34H,11-12H2,(H2,30,31,38)

InChi Key: IZHGETDBKCQWGN-UHFFFAOYSA-N