FKBP12 Antibody | 7789

FKBP12 Antibody | 7789 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rabbit: (100%)

Immunogen: FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12.
The immunogen is located within the first 50 amino acids of FKBP12.

Research Area: Homeostasis, Immunology

Tested Application: E, WB, ICC, IF

Application: FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: FKBP12 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 12 kDa
Observed: 12 kDa

Validation: N/A

Isoform: N/A

Purification: FKBP12 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: FKBP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: FKBP12 Antibody: FKBP1, PKC12, PKCI2, FKBP12, PPIASE, FKBP-12, FKBP-1A, FKBP1, Peptidyl-prolyl cis-trans isomerase FKBP1A, 12 kDa FK506-binding protein, PPIase FKBP1A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1) . FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2) . FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5) .