Flag-Tag Polyclonal Antibody | E-AB-20045

Flag-Tag Polyclonal Antibody | E-AB-20045 | Gentaur UK, US & Europe Distribution

Type: Tag Antibody

Synonyms: DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag

Application: WB

Reactivity: N/A

Host: Rabbit

Isotype: IgG

Reserch Areas: Tags and Cell Markers

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Affinity purification

Dilution: WB 1:5000-20000

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): N/A

Molecular Weight(Observed): N/A