flt-3-Ligand, murine recombinant | 4086

Flt3-Ligand belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant form is the transmembrane which is biologically active on the cell surface. The proteolytically cleaved transmembrane isoform generates a soluble form, which is also biologically active. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation. Recombinant murine flt3-Ligand is a 16.4 kDa protein containing 144 amino acid residues.

4086 | flt-3-Ligand murine recombinant DataSheet

Biomolecule/Target: Flt-3 Ligand

Synonyms: Fms-related tyrosine kinase 3 ligand

Alternates names: NOG

Taglines: A member of a small family of growth factors that stimulate the proliferation of hematopoietic cells

NCBI Gene ID #: 9241

NCBI Gene Symbol: NOG

Gene Source: Mouse

Accession #: Q13253

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to inhibit 5 ng/ml of BMP-4-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED for the effect is 0.05-0.08 µg/ml of Noggin

Results: 5-8 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 23.1 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized with no additives.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 mg/ml. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A