FLT3 Antibody | 7027

FLT3 Antibody | 7027 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: FLT3 antibody was raised against an 18 amino acid synthetic peptide near the center of human FLT3.
The immunogen is located within amino acids 340 - 390 of FLT3.

Research Area: Stem Cell

Tested Application: E, WB, IF

Application: FLT3 antibody can be used for detection of FLT3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of FLT3 are known to exist; this antibody will detect all isoforms.

Positive Control 1: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 109 kDa

Validation: N/A

Isoform: N/A

Purification: FLT3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: FLT3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: FLT3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: FLT3 Antibody: FLK2, STK1, CD135, FLK-2, FLK2, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: FLT3 Antibody: FLT3 is a member of the class III receptor tyrosine kinase family and its internal tandem duplication (ITD) is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. It is a membrane bound glycosylated protein that is expressed primarily in early hematopoietic progenitor cells. Upon binding its ligand FL, FLT3 activates multiple signaling pathways including the PI3K/Akt and Ras/MEK/MAPK pathways. In cells expressing FLT3-ITD, these pathways are constitutively on, resulting in abnormal cell growth and anti-apoptotic activity.