Flt3L/Fms-related tyrosine kinase 3 ligand | E21-A82

Flt3L/Fms-related tyrosine kinase 3 ligand | E21-A82 | Gentaur UK, US & Europe Distribution

Short Description: Recombinant Human Fms-like Tyrosine Kinase 3 Ligand is produced by our Mammalian expression system and the target gene encoding Thr27-Pro184 is expressed with a 6His tag at the C-terminus.

Shipping: Ambient

Formulation: Lyophilized from a 0.2 m filtered solution of 20mM TrisHClpH8.0.

Storage: Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 g /ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/g (1 IEU/g) as determined by LAL test.

Background: Fms-Related Tyrosine Kinase 3 Ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. Mature human Flt3 Ligand consists of a extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a transmembrane segment, and a cytoplasmic tail. Human and mouse Flt3 Ligand show cross-species activity. Flt3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts.It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1) . In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors by activiation of Flt 3 receptor.

Species: Human

Expression System: Human cells