Fluo-4 AM | B3146

Fluo-4 is a widely used green fluorescent, intracellular calcium (Ca²⁺) indicator. Fluo-4 AM is the membrane permeable form of the calcium indicator. Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. It binds to calcium with a Kd value of 355 nM and displays excitation/emission maxima of 490/515 nm, respectively. It is used to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.

Fluo-4 is a widely used green fluorescent, intracellular calcium (Ca²⁺) indicator. Fluo-4 AM is the membrane permeable form of the calcium indicator. Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. It binds to calcium with a Kd value of 355 nM and displays excitation/emission maxima of 490/515 nm, respectively. It is used to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.

B3146 | Fluo-4 AM DataSheet

Alternate Name/Synonyms: Calcium (Ca2⁺) indicator; Fluo-4 membrane permeable; Fluo-4 acetoxymethyl ester; Fluorescent Calcium indicator

Appearance: Red orange powder

Formulation:

CAS Number: 273221-67-3

Structure Available?: TRUE

Peptide sequence:

Salt Form: FALSE

Molecular Formula: C₅₁H₅₀F₂N₂O₂₃

Molecular Weight: 1097

Cell-Permeable?: TRUE

Purity: ≥ 95%

Solubilities: ~1 mg/ml in DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A membrane permeable fluorescent calcium indicator

MDL Number:

PubChem CID: 4060965

SMILES: CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi: InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3

InChi Key: QOMNQGZXFYNBNG-UHFFFAOYSA-N