FMR1 Antibody | 7707

FMR1 Antibody | 7707 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: FMR1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FMR1.
The immunogen is located within the last 50 amino acids of FMR1.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: FMR1 antibody can be used for detection of FMR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: FMR1 antibody is human, mouse and rat reactive. Multiple isoforms of FMR1 are known to exist.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 70 kDa
Observed: 74 kDa

Validation: N/A

Isoform: N/A

Purification: FMR1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: FMR1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: FMR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: FMR1 Antibody: Fragile X mental retardation protein 1, FMRP

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Fragile X syndrome is a frequent form of inherited mental retardation caused by functional loss of the fragile X mental retardation protein, FMR1, also known as FMRP (1) . FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm (2) . A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome (1) . Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1) (3) .