FOXK1 Antibody (C-term) [APR32745G]

FOXK1 Antibody (C-term) [APR32745G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This FOXK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-710 amino acids from the C-terminal region of human FOXK1.

Cellular Localisation: Nucleus. Cytoplasm. Note=Translocation to the nucleus is regulated by phosphorylation: phosphorylation by GSK3 (GSK3A or GSK3B) promotes interaction with 14-3-3 proteins and sequestration in the cytoplasm. Dephosphorylation promotes translocation to the nucleus (By similarity) . Accumulates in the nucleus upon viral infection (PubMed:25852164) . {ECO:0000250|UniProtKB:P42128, ECO:0000269|PubMed:25852164}

Molecular Weight: 75457

Clone: Polyclonal

Gene Name: FOXK1

Gene ID: 221937

Function: Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy (By similarity) . Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:17670796) . Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity) . Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity) . Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity) . Involved in mTORC1-mediated metabolic reprogramming: in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism (By similarity) . Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity) . Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle (By similarity) . Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population (By similarity) . Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation (By similarity) . Involved in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity) . Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity) . Represses myogenic differentiation by inhibiting MEFC activity (By similarity) . Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus (PubMed:25805136) . Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression (PubMed:25852164) .

Summary: Tissue Location: Expressed both developing and adult tissues (PubMed:15289879) . In adults, significant expression is seen in tumors of the brain, colon and lymph node (PubMed:15289879)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: FC, WB

Dilution: WB--1:500 FC--1:10~50

Synonyms: Forkhead box protein K1, Myocyte nuclear factor, MNF, FOXK1 {ECO:0000303|PubMed:15202027, ECO:0000303|PubMed:15289879}