FTCD Antibody | 45-057

FTCD Antibody | 45-057 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Pig

Homology: Expected Species Reactivity based on sequence homology: Rat, Cow

Immunogen: The immunogen for this antibody is: CLREQGRGKDQPGRL

Research Area: Homeostasis

Tested Application: E, WB, IF, IHC, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates and approx. 55-60kDa in Mouse and Pig Liver lysates, and also in preliminary testing of Rat Liver, Human Kidney and U2OS lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Pig NP_999440.1) . Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration: 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm, membranes and plasma membranes of HeLa and in the plasma membranes of HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Specificiy: Variants (NP_006648.1; NP_996848.1) encode the same protein.

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen

User Note: N/A

BACKGROUND: N/A