Fumarase Antibody | 45-641

Fumarase Antibody | 45-641 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog

Immunogen: The immunogen for this antibody is: C-HPNDHVNKSQSSND

Research Area: Cancer

Tested Application: E, WB, IHC, EIA

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2) . The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Specificiy: N/A

Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

User Note: N/A

BACKGROUND: N/A