GAD-65/67 (D572) polyclonal Antibody | BS1400

GAD-65/67 (D572) polyclonal Antibody | BS1400 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC IF

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background: GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GAD-65/67 (D572) polyclonal Antibody detects endogenous levels of GAD65 and GAD67 protein.

Molecular Weight: ~ 65, 67 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD 65; Glutamate decarboxylase 65 kDa isoform; GAD2; GAD-65; GAD 2; GAD-2; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD 67; Glutamate decarboxylase 67 kDa isoform; GAD1; GAD; GAD67; GAD-1; GAD 1

Immunogen: Synthetic peptide, corresponding to amino acids 540-590 of Human GAD-65.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: PI3K AKT signaling,Hedgehog Signaling,Hypoxia Signaling,Translational Contral Regulation of elF2,Regulation of Microtubule Dynamics,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,MTOR signaling,