GAI | DELLA protein GAI | AS11 1631

GAI | DELLA protein GAI | AS11 1631 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS13 2638 | Anti-GID2 | F-BOX protein GID2 (SLEEPY 1), rabbit antibodiesAS11 1630 | Anti-RGA | DELLA protein RGA, rabbit antibodiesAS11 1802 | Anti-RGA-like protein | DELLA protein RGL1, rabbit antibodiesAS11 1803 | Anti-RGA-like protein 2 | DELLA protein RGL2, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts

Molecular weight: 58, 9 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: N/A

Not reactive in: Brassica napus, Populus sp., Solanum lycopersicum

Additional Information: Homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here. GAI protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.

Background: GAI is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Less sensitive to GA. Synonymes:GRAS family protein 3, AtGRAS-3, Gibberelic acid-insensitive mutant protein, Restoration of growth on ammonia protein 2.

Reconstitution: For reconstitution add 25 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At1g14920

Category: Biosynthesis/regulation

Research Area: Plant Biology