Galectin-3, mouse recombinant | P1045

LGALS3, also known as galectin 3, is a member of the family of animal lectins, which selectively binds betagalactosidemresidues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. LGALS3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombinant mouse LGALS3 protein, used to Histag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1045 | Galectin-3 mouse recombinant DataSheet

Biomolecule/Target: Lgals3

Synonyms: Lectin, galactose binding, soluble 3, CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2

Alternates names: Mammotropin, Luterotropic hormone, Lutetropin, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R

Taglines: A protein involved in cell growth and differentiation, cell cycle, and apoptosis.

NCBI Gene ID #: 24683

NCBI Gene Symbol: PRL

Gene Source: Mouse

Accession #: P01237

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to agglutinate human red blood cells

Biological activity: The ED determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 cells was found to be in the range of 0.1-1.0 ng/ml.

Results: ED50 is 25 ug/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 22.5 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol,1mM DTT, 2mM EDTA

Reconstitution Instructions: Reconstitute in HO to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A