GALK1 Stable Knockout HEK293T Cell Line | T6153

GALK1 Stable Knockout HEK293T Cell Line | T6153

The Galactokinase 1 (GALK1) is major enzyme in the Leloir pathway, the primary pathway for galactose metabolism. Specifically, this enzyme modifies galactose with a phosphate group to generate galactose 1-phosphate which serves as a precursor for O-linked glycosylation. Deficiencies in GALK1 leads to Galactosemia: a congenital condition that causes galactose to accumulate in blood and tissue.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

18 - 28 hours

Seeding Density:

30,000 - 40,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Embryo

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available from abm

Preservation:

N/A

Quality Control:

1) Confirmed GALK1 knockout through sequencing (Figure S2). Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 4).

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C