GalNAc-T3, soluble fragment, Human Recombinant | P1216

Polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues. Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences. The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein. Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAc1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen. GalNAc-T3 is highly expressed in pancreas and testis, and weakly expressed in a few other organs such as placenta. The expression levels of GalNAc-T3 were found to be upregulated in highly differentiated types of adenocarcinoma cell lines as opposed to less differentiated types. Overexpression of GalNAc-T3 has also been found to promote pancreatic cancer cell growth. Mutations in GalNAc-T3 were found to be involved in familial tumoral calcinosis, which may be mediated through glycosylation of fibroblast growth factor 23, protecting it from proteolytic cleavage. GalNAc-T3 has a preference for mono- or diglycosylated substrates.

P1216 | GalNAc-T3 soluble fragment Human Recombinant DataSheet

Biomolecule/Target: GALNT3

Synonyms: Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GaNTase 3

Alternates names: Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GaNTase 3

Taglines: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis

NCBI Gene ID #: 25444

NCBI Gene Symbol: FGF9

Gene Source: Human

Accession #: P36364

Recombinant: Yes

Source: Insect cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by transfer of N-acetylgalatosamine (GalNac) from UDP-Gal to the peptide EA2, as measured with a phosphatase-coupled assay.

Biological activity: Specific activity >500 pmol/min/ug

Results: >500 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: N/A

Concentration: 466 ug/ml

Appearance: Liquid

Physical form description: Sterile filtered solution in 25 mM Tris pH7.5 and 150 mM NaCl

Reconstitution Instructions: N/A

Amino acid sequence: N/A