GalNAc-T5, soluble fragment, Human Recombinant | P1217

Polypeptide N-acetylgalactosaminyltransferase 5 (GalNAc-T5) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues, and was first cloned from rat. Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences. The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein. Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAc1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen. GalNAc-T5 expression pattern is highly regulated, and is most abundant in salivary glands. GalNAc-T5 has a preference for unglycosylated or monoglycosylated substrates.

P1217 | GalNAc-T5 soluble fragment Human Recombinant DataSheet

Biomolecule/Target: GALNT5

Synonyms: Polypeptide N-acetylgalactosaminyltransferase 5, Polypeptide GalNAc transferase 5, GaNTase 5

Alternates names: Polypeptide N-acetylgalactosaminyltransferase 5, Polypeptide GalNAc transferase 5, GaNTase 5

Taglines: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis

NCBI Gene ID #: 25444

NCBI Gene Symbol: FGF-9

Gene Source: Human

Accession #: P36364

Recombinant: Yes

Source: Insect cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by transfer of N-acetylgalatosamine (GalNac) from UDP-Gal to the peptide EA2, as measured with a phosphatase-coupled assay.

Biological activity: Specific activity >500 pmol/min/ug

Results: >500 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: N/A

Concentration: 477 ug/ml

Appearance: Liquid

Physical form description: Sterile filtered solution in 25 mM Tris pH 7.5 and 150 mM NaCl

Reconstitution Instructions: N/A

Amino acid sequence: N/A