Gamma Catenin Antibody [CTNG/1483] | 33-609

Gamma Catenin Antibody [CTNG/1483] | 33-609 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human protein was used as the immunogen for the Plakoglobin antibody.

Research Area: Signal Transduction

Tested Application: IF, WB, Flow, IHC-P

Application: Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
IHC (FFPE) : 0.1-0.2 ug/ml
The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: CTNG/1483

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3, JUP, CTNNG, DP3

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Plakoglobin, also known as junction plakoglobin or gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to beta-catenin. It is a cytoplasmic component of desmosomes and adherens junctions structures located within intercalated discs of cardiac muscle that function to anchor sarcomeres and join adjacent cells in cardiac muscle. Mutations in Plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. It links cadherins to the actin cytoskeleton. Plakoglobin binds to conserved regions of desmoglein and desmocollin at intracellular catenin-binding sites to assemble desmosomes. [Wiki]