Gamma-glutamyl-cysteine | AS11 1811

Gamma-glutamyl-cysteine | AS11 1811 | Gentaur UK, US & Europe Distribution

Immunogen: γ-glutamyl-cysteine (gamma-EC) linked by glutaraldehyde

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunocytochemistry (ICC), Immunogold (IG)

Related Products: AS06 186 | Anti-Gamma-ECS | Gamma glutamylcysteine synthase, rabbit antibodyother antibodies to proteins involved in response to heavy metalsPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 50 (ICC), (IG)

Molecular weight: N/A

Confirmed Reactivity: Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun

Predicted Reactivity: Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Immunogold labeling for electron microscopyBlock ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2) . Then treat the sections with the primary antibody (anti-γ-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.

Background: γ-glutamyl-cysteine is a precursor of glutathione and is synthesized by linkage of glutamate and cysteine by γ-glutamylcysteine synthetase. Synonymes:gamma-glutamyl-cysteine, γ-glutamyl-cysteine. Alternative names: γ-EC, γ-Glu-Cys.

Reconstitution: For reconstitution add 100 µl of sterile water per tube

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Oxidative stress

Research Area: Plant Biology