GAPDH Antibody [12D3H9] | PM-7571

GAPDH Antibody [12D3H9] | PM-7571 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Guinea pig: (100%) , Sheep: (93%) , Bovine: (93%) , Chicken: (93%) , Rabbit: (93%)

Immunogen: GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
The immunogen is located within amino acids 230 - 280 of GAPDH.

Research Area: Signal Transduction, Neuroscience, Homeostasis

Tested Application: E, WB

Application: GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Specificiy: GAPDH antibody is human, mouse, and rat reactive.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 37 kDa
Observed: 36 kDa

Validation: N/A

Isoform: N/A

Purification: GAPDH antibody is Protein A purified.

Clonality: Monoclonal

Clone: 12D3H9

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GAPDH antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) , an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.