GAPDH Antibody (HRP) | 3781-HRP

GAPDH Antibody (HRP) | 3781-HRP | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat, Rabbit, Chicken

Homology: N/A

Immunogen: HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

Research Area: Signal Transduction, Neuroscience, Homeostasis

Tested Application: E, WB

Application: HRP-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Specificiy: HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 37 kDa
Observed: 36 kDa

Validation: N/A

Isoform: N/A

Purification: HRP-GAPDH antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: HRP

Physical State: Liquid

Buffer: HRP-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% thimerosol.

Concentration: 1 mg/mL

Storage Condition: HRP-GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) , an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.